Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Myricetin exerts potent anticancer effects on human skin tumor cells

Wei Sun, Youming Tao , Daojiang Yu, Tianlan Zhao, Lijun Wu, Wenyuan Yu, Wenya Han

Department of Plastic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China;

For correspondence:- Youming Tao Email: tmilhollanaveka@yahoo.com Tel:+8651268282030

Accepted: 16 May 2018 Published: 30 June 2018

Citation: Sun W, Tao Y, Yu D, Zhao T, Wu L, Yu W, et al. Myricetin exerts potent anticancer effects on human skin tumor cells. Trop J Pharm Res 2018; 17(6):1067-1072 doi: 10.4314/tjpr.v17i6.13

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the anticancer activity of myricetin against skin cancer A431 cell lines.

Methods: Cell viability was determined by MTT and colony formation assays. Apoptosis was determined by DAPI and annexin V/PI staining. Cell cycle, ROS and MMP analysis were performed by flow cytometry. Cell migration and invasion were assessed by Boyden Chamber assay, while protein expression was determined using western blotting.

Results: Myricetin showed considerable anticancer activity against skin A431 cancer cell lines. However, lower cytotoxic effects were observed in normal FR2 cells. The anticancer activity of myricetin was due to ROS-prompted alterations in mitochondrial membrane potential and initiation of apoptotic cell death. The expressions of Bcl-2 and Bax were altered in response to myricetin treatment. Myricetin also induced cell cycle arrest and suppressed the migration and invasion of A431 cells.

Conclusion: These results suggest that myricetin may be an important lead molecule for the development of a suitable treatment of skin cancer.

Keywords: Skin carcinoma, ROS, Apoptosis, Myricetin, Cell migration